› 8j60

Electron Microscopy
3.39Å resolution

Structural and mechanistic insight into ribosomal ITS2 RNA processing by nuclease-kinase machinery

Released:
DOI: 10.2210/pdb8j60/pdb
PDB entry 8j60 coloured by chain, front view.
PDB entry 8j60 coloured by chain, side view.
PDB entry 8j60 coloured by chain, top view.
Source organism: Cyberlindnera jadinii
Primary publication:
Structural and mechanistic insights into ribosomal ITS2 RNA processing by nuclease-kinase machinery.
Chen J, Chen H, Li S, Lin X, Hu R, Zhang K, Liu L
Elife 12 (2024)
PMID: 38180340
Related structures: EMD-33735

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-238814 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Polynucleotide 5'-hydroxyl-kinase GRC3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 610 amino acids
Theoretical weight: 69.61 KDa
Source organism: Cyberlindnera jadinii
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A0H5C3P3 (Residues: 1-610; Coverage: 100%)
Gene names: BN1211_2636, CYBJADRAFT_171070, GRC3
Sequence domains: mRNA cleavage and polyadenylation factor CLP1 P-loop
LAS1 protein Chains: C, D
Molecule details ›
Chains: C, D
Length: 421 amino acids
Theoretical weight: 49.41 KDa
Source organism: Cyberlindnera jadinii
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A0H5CBH3 (Residues: 1-421; Coverage: 100%)
Gene names: BN1211_1791, LAS1
Sequence domains: Las1-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.39Å
Relevant EMDB volumes: EMD-33735
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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