› 8j6r

Electron Microscopy
2.76Å resolution

Cryo-EM structure of the MK-6892-bound human HCAR2-Gi1 complex

Released:
DOI: 10.2210/pdb8j6r/pdb
PDB entry 8j6r coloured by chain, front view.
PDB entry 8j6r coloured by chain, side view.
PDB entry 8j6r coloured by chain, top view.
Source organisms:
Primary publication:
Orthosteric and allosteric modulation of human HCAR2 signaling complex.
Mao C, Gao M, Zang SK, Zhu Y, Shen DD, Chen LN, Yang L, Wang Z, Zhang H, Wang WW, Shen Q, Lu Y, Ma X, Zhang Y
OpenAccess logo Nat Commun 14 7620 (2023)
PMID: 37993467
Related structures: EMD-36012

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-273988 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 352 amino acids
Theoretical weight: 40.23 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63096 (Residues: 3-354; Coverage: 99%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 339 amino acids
Theoretical weight: 37.29 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 58 amino acids
Theoretical weight: 6.38 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 5-62; Coverage: 82%)
Gene name: GNG2
Sequence domains: GGL domain
single Fab chain (scFv16) Chain: S
Molecule details ›
Chain: S
Length: 250 amino acids
Theoretical weight: 26.61 KDa
Source organism: synthetic construct
Expression system: Trichoplusia ni
Hydroxycarboxylic acid receptor 2 Chain: R
Molecule details ›
Chain: R
Length: 295 amino acids
Theoretical weight: 34.37 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8TDS4 (Residues: 8-301; Coverage: 81%)
Gene names: GPR109A, HCA2, HCAR2, HM74A, NIACR1
Sequence domains: 7 transmembrane receptor (rhodopsin family)

Ligands and Environments

2 bound ligands:
Ligand FI7 1 x FI7
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.76Å
Relevant EMDB volumes: EMD-36012
Expression systems:
  • Spodoptera frugiperda
  • Trichoplusia ni

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