8j7s

Electron Microscopy
2.84Å resolution

Structure of the SPARTA complex

Released:
Source organism: Maribacter polysiphoniae
Primary publication:
Cryo-EM structure of the ssDNA-activated SPARTA complex.
Cell Res 33 731-734 (2023)
PMID: 37491603
Related structures: EMD-36047

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexadecamer (preferred)
PDBe Complex ID:
PDB-CPX-269209 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct DNA molecule
1 distinct RNA molecule
Macromolecules (4 distinct):
Piwi domain-containing protein Chains: A, E, I, M
Molecule details ›
Chains: A, E, I, M
Length: 506 amino acids
Theoretical weight: 57.98 KDa
Source organism: Maribacter polysiphoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A316E3U6 (Residues: 1-506; Coverage: 100%)
Gene name: LX92_01809
TIR domain-containing protein Chains: B, F, J, N
Molecule details ›
Chains: B, F, J, N
Length: 418 amino acids
Theoretical weight: 49.4 KDa
Source organism: Maribacter polysiphoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A316E683 (Residues: 1-418; Coverage: 93%)
Gene name: LX92_01810
Sequence domains: TIR domain
DNA (5'-D(P*TP*AP*AP*TP*AP*GP*AP*TP*TP*AP*GP*AP*GP*CP*CP*GP*TP*CP*AP*AP*TP*AP*GP*A)-3') Chains: D, H, L, P
RNA (5'-R(P*UP*GP*AP*CP*GP*GP*CP*UP*CP*UP*AP*AP*UP*CP*UP*AP*UP*UP*A)-3') Chains: C, G, K, O

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.84Å
Relevant EMDB volumes: EMD-36047
Expression system: Escherichia coli