› 8j8c

Electron Microscopy
2.69Å resolution

Cryo-EM structure of Asfv topoisomerase 2 - apo conformer IIIb

Released:
DOI: 10.2210/pdb8j8c/pdb
PDB entry 8j8c coloured by chain, front view.
PDB entry 8j8c coloured by chain, side view.
PDB entry 8j8c coloured by chain, top view.
Source organism: African swine fever virus
Primary publication:
A unified view on enzyme catalysis by cryo-EM study of a DNA topoisomerase.
Chang CM, Wang SC, Wang CH, Pang AH, Yang CH, Chang YK, Wu WJ, Tsai MD
OpenAccess logo Commun Chem 7 45 (2024)
PMID: 38418525
Related structures: EMD-36067

Function and Biology Details

Reaction catalysed: 
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-274405 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA topoisomerase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 1197 amino acids
Theoretical weight: 136.42 KDa
Source organism: African swine fever virus
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q00942 (Residues: 1-1192; Coverage: 100%)
Gene names: Ba71V-112, P1192R, TOP
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.69Å
Relevant EMDB volumes: EMD-36067
Expression system: Saccharomyces cerevisiae

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