8j8h

Electron Microscopy
3.4Å resolution

SPARTA monomer bound with guide-target, state 2

Released:

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-268914 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct DNA molecule
1 distinct RNA molecule
Macromolecules (4 distinct):
Piwi domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 541 amino acids
Theoretical weight: 61.86 KDa
Source organism: Thermoflavifilum thermophilum
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A1I7NFD7 (Residues: 1-507; Coverage: 100%)
Gene name: SAMN05660895_1671
TIR domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 484 amino acids
Theoretical weight: 56.81 KDa
Source organism: Thermoflavifilum thermophilum
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A1I7NFG5 (Residues: 1-450; Coverage: 100%)
Gene name: SAMN05660895_1670
Sequence domains: TIR domain
DNA (25-MER) Chain: F
Molecule details ›
Chain: F
Length: 25 nucleotides
Theoretical weight: 7.68 KDa
RNA (5'-R(P*UP*GP*AP*CP*GP*GP*CP*UP*CP*UP*AP*AP*UP*CP*UP*AP*UP*UP*AP*GP*U)-3') Chain: E
Molecule details ›
Chain: E
Length: 21 nucleotides
Theoretical weight: 6.65 KDa

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-36070
Expression system: Escherichia coli BL21