› 8j8p

X-ray diffraction
2.7Å resolution

Structure of the four-component Paf1 complex from Saccharomyces eubayanus

Released:
DOI: 10.2210/pdb8j8p/pdb
PDB entry 8j8p coloured by chain, front view.
PDB entry 8j8p coloured by chain, side view.
PDB entry 8j8p coloured by chain, top view.
Source organism: Saccharomyces eubayanus
Primary publication:
Structural Basis of the Transcriptional Elongation Factor Paf1 Core Complex from Saccharomyces eubayanus.
Qin Y, Zhou Y, Cao Y, Ren Y, Deng P, Jiang J, Wang Z
OpenAccess logo Int J Mol Sci 24 (2023)
PMID: 37240075

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-216086 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
CTR9-like protein Chain: C
Molecule details ›
Chain: C
Length: 908 amino acids
Theoretical weight: 104.81 KDa
Source organism: Saccharomyces eubayanus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0L8RHL9 (Residues: 1-907; Coverage: 84%)
Gene name: DI49_2263
Sequence domains: Tetratricopeptide repeat
Cell division control protein 73 C-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 81 amino acids
Theoretical weight: 8.49 KDa
Source organism: Saccharomyces eubayanus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0L8RF82 (Residues: 153-233; Coverage: 21%)
Gene name: DI49_3914
PAF1-like protein Chain: P
Molecule details ›
Chain: P
Length: 111 amino acids
Theoretical weight: 12.7 KDa
Source organism: Saccharomyces eubayanus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0L8RM45 (Residues: 1-110; Coverage: 25%)
Gene name: DI49_1302
Plus3 domain-containing protein Chain: R
Molecule details ›
Chain: R
Length: 77 amino acids
Theoretical weight: 9.37 KDa
Source organism: Saccharomyces eubayanus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0L8RIY1 (Residues: 494-570; Coverage: 14%)
Gene name: DI49_1738

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P21
Unit cell:
a: 71.775Å b: 88.48Å c: 127.775Å
α: 90° β: 97.272° γ: 90°
R-values:
R R work R free
0.227 0.225 0.262
Expression system: Escherichia coli

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