› 8j97

Electron Microscopy
3.2Å resolution

Structure of Muscarinic receptor (M2R) in complex with beta-arrestin1 (Local refine, cross-linked)

Released:
DOI: 10.2210/pdb8j97/pdb
PDB entry 8j97 coloured by chain, front view.
PDB entry 8j97 coloured by chain, side view.
PDB entry 8j97 coloured by chain, top view.
Source organisms:
Primary publication:
Molecular insights into atypical modes of β-arrestin interaction with seven transmembrane receptors.
Maharana J, Sano FK, Sarma P, Yadav MK, Duan L, Stepniewski TM, Chaturvedi M, Ranjan A, Singh V, Saha S, Mahajan G, Chami M, Shihoya W, Selent J, Chung KY, Banerjee R, Nureki O, Shukla AK
Science 383 101-108 (2024)
PMID: 38175886
Related structures: EMD-36090

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-237568 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Beta-arrestin-1 Chain: A
Molecule details ›
Chain: A
Length: 353 amino acids
Theoretical weight: 39.66 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P17870 (Residues: 5-357; Coverage: 84%)
Gene name: ARRB1
Sequence domains:
Fab30 Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 117 amino acids
Theoretical weight: 12.86 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Fab30 Light Chain Chain: L
Molecule details ›
Chain: L
Length: 106 amino acids
Theoretical weight: 11.5 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Muscarinic acetylcholine receptor M2 Chain: V
Molecule details ›
Chain: V
Length: 9 amino acids
Theoretical weight: 1.23 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P08172 (Residues: 305-313; Coverage: 2%)
Gene name: CHRM2

Ligands and Environments

No bound ligands
2 modified residues:
Ligand TPO 2 x TPO
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-36090
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

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