› 8j9p

Electron Microscopy
3.4Å resolution

SPARTA dimer bound with guide-target

Released:
DOI: 10.2210/pdb8j9p/pdb
PDB entry 8j9p coloured by chain, front view.
PDB entry 8j9p coloured by chain, side view.
PDB entry 8j9p coloured by chain, top view.
Source organism: Thermoflavifilum thermophilum
Primary publication:
Auto-inhibition and activation of a short Argonaute-associated TIR-APAZ defense system.
Guo L, Huang P, Li Z, Shin YC, Yan P, Lu M, Chen M, Xiao Y
Nat Chem Biol (2023)
PMID: 37932527
Related structures: EMD-36114

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-268917 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct DNA molecule
1 distinct RNA molecule
Macromolecules (4 distinct):
Piwi domain-containing protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 507 amino acids
Theoretical weight: 58.3 KDa
Source organism: Thermoflavifilum thermophilum
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A1I7NFD7 (Residues: 1-507; Coverage: 100%)
Gene name: SAMN05660895_1671
TIR domain-containing protein Chains: B, D
Molecule details ›
Chains: B, D
Length: 450 amino acids
Theoretical weight: 53.26 KDa
Source organism: Thermoflavifilum thermophilum
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A1I7NFG5 (Residues: 1-450; Coverage: 100%)
Gene name: SAMN05660895_1670
Sequence domains: TIR domain
DNA (25-MER) Chains: F, H
RNA (5'-R(P*UP*GP*AP*CP*GP*GP*CP*UP*CP*UP*AP*AP*UP*CP*UP*AP*UP*UP*AP*GP*U)-3') Chains: E, G

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-36114
Expression system: Escherichia coli BL21

Quick links

Citations

3 citation in other articles

Capturing receptor states with glue.
Bernhard et al. (2024)
2 more