› 8j9z

Electron Microscopy
3.13Å resolution

cryo-EM structure of viral topoisomerase in conformation 2

Released:
DOI: 10.2210/pdb8j9z/pdb
PDB entry 8j9z coloured by chain, front view.
PDB entry 8j9z coloured by chain, side view.
PDB entry 8j9z coloured by chain, top view.
Source organism: African swine fever virus LIS57
Primary publication:
Cryo-EM structures of African swine fever virus topoisomerase.
Zhao Y, Kuang W, An Q, Li J, Wang Y, Deng Z
OpenAccess logo mBio e0122823 (2023)
PMID: 37610250
Related structures: EMD-36120

Function and Biology Details

Reaction catalysed: 
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-226598 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA topoisomerase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 1192 amino acids
Theoretical weight: 135.69 KDa
Source organism: African swine fever virus LIS57
Expression system: Komagataella pastoris
UniProt:
  • Canonical: A0A2X0THW2 (Residues: 1-1192; Coverage: 100%)
Gene names: ASFV-Georgia_4-136, P1192R, P1192R CDS
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.13Å
Relevant EMDB volumes: EMD-36120
Expression system: Komagataella pastoris

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