› 8ja0

Electron Microscopy
3.52Å resolution

Cryo-EM structure of the NmeCas9-sgRNA-AcrIIC4 ternary complex

Released:
DOI: 10.2210/pdb8ja0/pdb
PDB entry 8ja0 coloured by chain, front view.
PDB entry 8ja0 coloured by chain, side view.
PDB entry 8ja0 coloured by chain, top view.
Source organisms:
Entry authors: Yin H, Li Z, Yu GM, Li XZ
Related structures: EMD-36123

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-224645 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
CRISPR-associated endonuclease Cas9 Chain: A
Molecule details ›
Chain: A
Length: 1082 amino acids
Theoretical weight: 124.61 KDa
Source organism: Neisseria meningitidis
Expression system: Escherichia coli
UniProt:
  • Canonical: C9X1G5 (Residues: 1-1082; Coverage: 100%)
Gene names: NMV_1993, cas9
Sequence domains:
Uncharacterized protein Chain: D
Molecule details ›
Chain: D
Length: 88 amino acids
Theoretical weight: 9.99 KDa
Source organism: Haemophilus parainfluenzae
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A377JKY9 (Residues: 1-88; Coverage: 100%)
Gene names: NCTC10672_00033, NCTC10672_02354
RNA (117-MER) Chain: B
Molecule details ›
Chain: B
Length: 117 nucleotides
Theoretical weight: 37.37 KDa
Sequence domains: CRISPR RNA direct repeat element

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.52Å
Relevant EMDB volumes: EMD-36123
Expression system: Escherichia coli

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