› 8jal

Electron Microscopy
3.3Å resolution

Structure of CRL2APPBP2 bound with RxxGP degron (dimer)

Released:
DOI: 10.2210/pdb8jal/pdb
PDB entry 8jal coloured by chain, front view.
PDB entry 8jal coloured by chain, side view.
PDB entry 8jal coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis for C-degron recognition by CRL2APPBP2 ubiquitin ligase.
Zhao S, Olmayev-Yaakobov D, Ru W, Li S, Chen X, Zhang J, Yao X, Koren I, Zhang K, Xu C
Proc Natl Acad Sci U S A 120 e2308870120 (2023)
PMID: 37844242
Related structures: EMD-36129

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-232475 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Amyloid protein-binding protein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 585 amino acids
Theoretical weight: 66.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92624 (Residues: 1-585; Coverage: 100%)
Gene names: APPBP2, KIAA0228, PAT1
Sequence domains:
Cullin-2 Chains: E, I
Molecule details ›
Chains: E, I
Length: 745 amino acids
Theoretical weight: 87.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13617 (Residues: 2-745; Coverage: 100%)
Gene name: CUL2
Sequence domains:
Elongin-B Chains: C, G
Molecule details ›
Chains: C, G
Length: 118 amino acids
Theoretical weight: 13.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15370 (Residues: 1-118; Coverage: 100%)
Gene names: ELOB, TCEB2
Sequence domains: Ubiquitin family
Elongin-C Chains: D, H
Molecule details ›
Chains: D, H
Length: 112 amino acids
Theoretical weight: 12.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15369 (Residues: 1-112; Coverage: 100%)
Gene names: ELOC, TCEB1
Sequence domains: Skp1 family, tetramerisation domain
LEU-THR-ARG-ASN-LYS-GLY-PRO Chains: F, S
Molecule details ›
Chains: F, S
Length: 11 amino acids
Theoretical weight: 1.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-36129
Expression system: Escherichia coli

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