› 8jat

X-ray diffraction
3.2Å resolution

Crystal structure of the 3-ketodihydrosphingosine reductase TSC10 from Cryptococcus neoformans

Released:
DOI: 10.2210/pdb8jat/pdb
PDB entry 8jat coloured by chain, front view.
PDB entry 8jat coloured by chain, side view.
PDB entry 8jat coloured by chain, top view.
Source organism: Cryptococcus neoformans var. neoformans JEC21
Primary publication:
Crystal structure of the 3-ketodihydrosphingosine reductase TSC10 from Cryptococcus neoformans.
Zhao P, Zhuang Z, Guan X, Yang J, Wang W, Kuang Z
Biochem Biophys Res Commun 670 73-78 (2023)
PMID: 37285720

Function and Biology Details

Reaction catalysed: 
Sphinganine + NADP(+) = 3-dehydrosphinganine + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-218518 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-ketodihydrosphingosine reductase TSC10 Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 29.77 KDa
Source organism: Cryptococcus neoformans var. neoformans JEC21
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CR36 (Residues: 29-293; Coverage: 79%)
Gene names: CNG00270, TSC10
Sequence domains: short chain dehydrogenase

Ligands and Environments


Cofactor: Ligand NDP 1 x NDP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL02U1
Spacegroup: P6122
Unit cell:
a: 111.311Å b: 111.311Å c: 80.399Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.213 0.21 0.282
Expression system: Escherichia coli

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