› 8jdh

X-ray diffraction
1.77Å resolution

Crystal structure of anti-CRISPR AcrIF25

Released:
DOI: 10.2210/pdb8jdh/pdb
PDB entry 8jdh coloured by chain, front view.
PDB entry 8jdh coloured by chain, side view.
PDB entry 8jdh coloured by chain, top view.
Source organism: Alcanivorax sp. KX64203
Primary publication:
An anti-CRISPR that pulls apart a CRISPR-Cas complex.
Trost CN, Yang J, Garcia B, Hidalgo-Reyes Y, Fung BCM, Wang J, Lu WT, Maxwell KL, Wang Y, Davidson AR
Nature (2024)
PMID: 38961300

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-244855 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AcrIF25 Chains: A, B
Molecule details ›
Chains: A, B
Length: 166 amino acids
Theoretical weight: 18.39 KDa
Source organism: Alcanivorax sp. KX64203
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: C2
Unit cell:
a: 85.953Å b: 52.289Å c: 85.121Å
α: 90° β: 105.91° γ: 90°
R-values:
R R work R free
0.169 0.168 0.192
Expression system: Escherichia coli BL21(DE3)

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