› 8jeb

X-ray diffraction
1.3Å resolution

Crystal structure of CGL1 from Crassostrea gigas, mannotetraose-bound form (CGL1/Man(alpha)1-2Man(alpha)1-2Man(alpha)1-6Man)

Released:
DOI: 10.2210/pdb8jeb/pdb
PDB entry 8jeb coloured by chain, front view.
PDB entry 8jeb coloured by chain, side view.
PDB entry 8jeb coloured by chain, top view.
Source organism: Crassostrea gigas
Primary publication:
Mannose oligosaccharide recognition of CGL1, a mannose-specific lectin containing DM9 motifs from Crassostrea gigas, revealed by X-ray crystallographic analysis.
Hatakeyama T, Masuda K, Kudo M, Tanaka K, Takeuchi A, Unno H
J Biochem (2023)
PMID: 37793172

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-516466 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Natterin-3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 142 amino acids
Theoretical weight: 15.45 KDa
Source organism: Crassostrea gigas
UniProt:
  • Canonical: K1QRB6 (Residues: 2-143; Coverage: 99%)
Gene names: CGI_10011001, CGI_10018577
Sequence domains: DM9 repeat

Ligands and Environments

Carbohydrate polymer : NEW Components: MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: MAN
Carbohydrate polymer
2 bound ligands:
Ligand ACY 5 x ACY
Ligand MG 5 x MG
1 modified residue:
Ligand AYA 2 x AYA

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P21212
Unit cell:
a: 76.4Å b: 82.495Å c: 44.201Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.157 0.156 0.174

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