› 8jei

Electron Microscopy
2.73Å resolution

Cryo-EM Structure of the compuond 5c-HCAR3-Gi complex

Released:
DOI: 10.2210/pdb8jei/pdb
PDB entry 8jei coloured by chain, front view.
PDB entry 8jei coloured by chain, side view.
PDB entry 8jei coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for ligand recognition of the human hydroxycarboxylic acid receptor HCAR3.
Ye F, Pan X, Zhang Z, Xiang X, Li X, Zhang B, Ning P, Liu A, Wang Q, Gong K, Li J, Zhu L, Qian C, Chen G, Du Y
Cell Rep 43 114895 (2024)
PMID: 39427321
Related structures: EMD-36189

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-281614 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: C
Molecule details ›
Chain: C
Length: 351 amino acids
Theoretical weight: 40.15 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63096 (Residues: 4-354; Coverage: 99%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 337 amino acids
Theoretical weight: 37.07 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 4-340; Coverage: 99%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 56 amino acids
Theoretical weight: 6.22 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 8-63; Coverage: 79%)
Gene name: GNG2
Sequence domains: GGL domain
scFv16 Chain: S
Molecule details ›
Chain: S
Length: 247 amino acids
Theoretical weight: 26.34 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Hydroxycarboxylic acid receptor 3 Chain: A
Molecule details ›
Chain: A
Length: 387 amino acids
Theoretical weight: 44.53 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P49019 (Residues: 1-387; Coverage: 100%)
Gene names: GPR109B, HCA3, HCAR3, HM74B, NIACR2
Sequence domains: 7 transmembrane receptor (rhodopsin family)

Ligands and Environments

1 bound ligand:
Ligand CW3 1 x CW3
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.73Å
Relevant EMDB volumes: EMD-36189
Expression system: Spodoptera frugiperda

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