8jer

Electron Microscopy
3.45Å resolution

Structure of Acipimox-GPR109A-G protein complex

Released:

Function and Biology Details

Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-272404 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(o) subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 240 amino acids
Theoretical weight: 27.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09471 (Residues: 6-57, 183-354; Coverage: 61%)
Gene name: GNAO1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 350 amino acids
Theoretical weight: 38.53 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Hydroxycarboxylic acid receptor 2; Muscarinic acetylcholine receptor M4 Chain: R
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
ScFv16 Antibody fragment Chain: H

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.45Å
Relevant EMDB volumes: EMD-36193
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda