› 8jfz

Electron Microscopy
3.5Å resolution

Cryo-EM structure of Na+,K+-ATPase in the E1.Mg2+ state.

Released:
DOI: 10.2210/pdb8jfz/pdb
PDB entry 8jfz coloured by chain, front view.
PDB entry 8jfz coloured by chain, side view.
PDB entry 8jfz coloured by chain, top view.
Source organism: Squalus acanthias
Primary publication:
Crystal structures of Na+ ,K+ -ATPase reveal the mechanism that converts the K+ -bound form to Na+ -bound form and opens and closes the cytoplasmic gate.
Kanai R, Vilsen B, Cornelius F, Toyoshima C
FEBS Lett (2023)
PMID: 37357620
Related structures: EMD-36220

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111292 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (7 distinct):
Sodium/potassium-transporting ATPase subunit beta Chains: B, D
Molecule details ›
Chains: B, D
Length: 305 amino acids
Theoretical weight: 35.18 KDa
Source organism: Squalus acanthias
UniProt:
  • Canonical: C4IX13 (Residues: 1-305; Coverage: 100%)
Gene name: atp1b1
Sequence domains: Sodium / potassium ATPase beta chain
Sodium/potassium-transporting ATPase subunit alpha Chains: A, C
Molecule details ›
Chains: A, C
Length: 1028 amino acids
Theoretical weight: 113.31 KDa
Source organism: Squalus acanthias
UniProt:
  • Canonical: Q4H132 (Residues: 1-1028; Coverage: 100%)
Gene name: atn1
Sequence domains:
FXYD domain-containing ion transport regulator Chains: E, G
Molecule details ›
Chains: E, G
Length: 94 amino acids
Theoretical weight: 10.2 KDa
Source organism: Squalus acanthias
UniProt:
  • Canonical: Q70Q12 (Residues: 1-94; Coverage: 100%)
Gene name: fxyd10
Sequence domains: ATP1G1/PLM/MAT8 family

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand CLR 8 x CLR
Ligand PCW 20 x PCW
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-36220

Quick links

Citations

2 citation in other articles

Effect of Cations on ATP Binding to the N-domain of Na+, K+-ATPase.
Ramírez-Alonso et al. (2024)
1 more

1 mention without citation

Na+/K+-ATPase: More than an Electrogenic Pump.
Contreras et al. (2024)