› 8jg3

X-ray diffraction
2Å resolution

Biosynthetic thiolase from Clostridium kluyveri

Released:
DOI: 10.2210/pdb8jg3/pdb
PDB entry 8jg3 coloured by chain, front view.
PDB entry 8jg3 coloured by chain, side view.
PDB entry 8jg3 coloured by chain, top view.
Source organism: Clostridium kluyveri
Entry authors: Kim E-J, Seo H, Kim K-J

Function and Biology Details

Reaction catalysed: 
(1a) acetyl-CoA + [acetyl-CoA C-acetyltransferase]-L-cysteine = [acetyl-CoA C-acetyltransferase]-S-acetyl-L-cysteine + CoA
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-244011 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetyl-CoA acetyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 401 amino acids
Theoretical weight: 42.62 KDa
Source organism: Clostridium kluyveri
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B9DX69 (Residues: 1-393; Coverage: 100%)
Gene name: CKR_3261
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P3121
Unit cell:
a: 73.568Å b: 73.568Å c: 273.996Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.209 0.24
Expression system: Escherichia coli BL21(DE3)

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