› 8jgg

Electron Microscopy
3Å resolution

CryoEM structure of Gi-coupled MRGPRX1 with peptide agonist BAM8-22

Released:
DOI: 10.2210/pdb8jgg/pdb
PDB entry 8jgg coloured by chain, front view.
PDB entry 8jgg coloured by chain, side view.
PDB entry 8jgg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Ligand recognition and G protein coupling of the human itch receptor MRGPRX1.
Guo L, Zhang Y, Fang G, Tie L, Zhuang Y, Xue C, Liu Q, Zhang M, Zhu K, You C, Xu P, Yuan Q, Zhang C, Liu L, Rong N, Peng S, Liu Y, Wang C, Luo X, Lv Z, Kang D, Yu X, Zhang C, Jiang Y, Dong X, Zhou J, Liu Z, Yang F, Eric Xu H, Sun JP
OpenAccess logo Nat Commun 14 5004 (2023)
PMID: 37591889
Related structures: EMD-36233

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-238641 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 60 amino acids
Theoretical weight: 6.63 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 5-64; Coverage: 85%)
Gene name: GNG2
Sequence domains: GGL domain
Leu-enkephalin Chain: L
Molecule details ›
Chain: L
Length: 11 amino acids
Theoretical weight: 1.6 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01210 (Residues: 220-230; Coverage: 5%)
Gene name: PENK
Single-Chain Fragment Variable 16 Chain: S
Molecule details ›
Chain: S
Length: 285 amino acids
Theoretical weight: 30.36 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 40.42 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63096 (Residues: 1-354; Coverage: 100%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 352 amino acids
Theoretical weight: 38.56 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Mas-related G-protein coupled receptor member X1 Chain: R
Molecule details ›
Chain: R
Length: 322 amino acids
Theoretical weight: 36.28 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q96LB2 (Residues: 1-322; Coverage: 100%)
Gene names: MRGPRX1, MRGX1, SNSR3, SNSR4
Sequence domains: 7 transmembrane receptor (rhodopsin family)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-36233
Expression systems:
  • Spodoptera frugiperda
  • Not provided

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