› 8jgp

X-ray diffraction
3.02Å resolution

Crystal structure of Deinococcus radiodurans exopolyphosphatase complexed with pyrophosphate

Released:
DOI: 10.2210/pdb8jgp/pdb
PDB entry 8jgp coloured by chain, front view.
PDB entry 8jgp coloured by chain, side view.
PDB entry 8jgp coloured by chain, top view.
Source organism: Deinococcus radiodurans R1
Primary publication:
Structural Evolution of Bacterial Polyphosphate Degradation Enzyme for Phosphorus Cycling.
Dai S, Wang B, Ye R, Zhang D, Xie Z, Yu N, Cai C, Huang C, Zhao J, Zhang F, Hua Y, Zhao Y, Zhou R, Tian B
OpenAccess logo Adv Sci (Weinh) e2309602 (2024)
PMID: 38682481

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-577055 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Exopolyphosphatase Chain: A
Molecule details ›
Chain: A
Length: 535 amino acids
Theoretical weight: 56.83 KDa
Source organism: Deinococcus radiodurans R1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9RYW9 (Residues: 1-515; Coverage: 100%)
Gene name: DR_A0185
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand PPV 1 x PPV
Ligand MN 1 x MN
Ligand SO4 2 x SO4
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P61
Unit cell:
a: 168.25Å b: 168.25Å c: 39.84Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.267 0.264 0.317
Expression system: Escherichia coli

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Citations

1 mention without citation

Structural Evolution of Bacterial Polyphosphate Degradation Enzyme for Phosphorus Cycling.
Dai et al. (2024)