8jh2

Electron Microscopy
5.7Å resolution

RNA polymerase II elongation complex bound with Elf1, Spt4/5 and foreign DNA, stalled at SHL(-1) of the nucleosome

Released:

Function and Biology Details

Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 28-mer (preferred)
PDBe Complex ID:
PDB-CPX-270089 (preferred)
Entry contents:
19 distinct polypeptide molecules
4 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (24 distinct):
DNA-directed RNA polymerase subunit Chain: A
DNA-directed RNA polymerase subunit beta Chain: B
Molecule details ›
Chain: B
Length: 1227 amino acids
Theoretical weight: 139.75 KDa
Source organism: Komagataella phaffii
UniProt:
  • Canonical: C4QZQ7 (Residues: 1-1227; Coverage: 100%)
Gene name: PAS_chr2-1_0125
Sequence domains:
DNA-directed RNA polymerase RpoA/D/Rpb3-type domain-containing protein Chain: C
RNA polymerase Rpb4/RPC9 core domain-containing protein Chain: D
DNA-directed RNA polymerases I, II, and III subunit RPABC1 Chain: E
RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III Chain: F
DNA-directed RNA polymerase subunit Chain: G
DNA-directed RNA polymerases I, II, and III subunit RPABC3 Chain: H
DNA-directed RNA polymerase subunit Chain: I
DNA-directed RNA polymerases I, II, and III subunit RPABC5 Chain: J
DNA-directed RNA polymerase RBP11-like dimerisation domain-containing protein Chain: K
RNA polymerase subunit ABC10-alpha Chain: L
Transcription elongation factor 1 homolog Chain: M
Transcription elongation factor SPT4 Chain: V
Transcription elongation factor SPT5 Chain: W
Histone H3.3 Chains: a, e
Histone H4 Chains: b, f
Histone H2A type 1-B/E Chains: c, g
Histone H2B type 1-J Chains: d, h
DNA (40-MER) Chain: 0
DNA (40-MER) Chain: 1
DNA (218-MER) Chain: N
DNA (218-MER) Chain: T
RNA (5'-R(P*CP*CP*UP*GP*GP*UP*GP*UP*CP*UP*UP*GP*GP*GP*UP*G)-3') Chain: P

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 5.7Å
Relevant EMDB volumes: EMD-36251
Expression system: Escherichia coli