› 8jhb

Electron Microscopy
3.3Å resolution

FZD6 Gs complex

Released:
DOI: 10.2210/pdb8jhb/pdb
PDB entry 8jhb coloured by chain, front view.
PDB entry 8jhb coloured by chain, side view.
PDB entry 8jhb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A framework for Frizzled-G protein coupling and implications to the PCP signaling pathways.
Zhang Z, Lin X, Wei L, Wu Y, Xu L, Wu L, Wei X, Zhao S, Zhu X, Xu F
OpenAccess logo Cell Discov 10 3 (2024)
PMID: 38182578
Related structures: EMD-36261

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-238913 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas Chain: A
Molecule details ›
Chain: A
Length: 270 amino acids
Theoretical weight: 31.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5JWF2 (Residues: 655-707, 847-896, 907-1037; Coverage: 23%)
  • Best match: Q5JWF2-3 (Residues: 655-690, 529-540, 541-551, 552-576)
Gene names: GNAS, GNAS1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 340 amino acids
Theoretical weight: 37.42 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 1-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: C
Molecule details ›
Chain: C
Length: 74 amino acids
Theoretical weight: 8.42 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 5-71; Coverage: 94%)
Gene name: GNG2
Sequence domains: GGL domain
Nb35 Chain: N
Molecule details ›
Chain: N
Length: 135 amino acids
Theoretical weight: 14.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Frizzled-6 Chain: R
Molecule details ›
Chain: R
Length: 537 amino acids
Theoretical weight: 61.91 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O60353 (Residues: 19-525; Coverage: 74%)
  • Best match: O60353-2 (Residues: 1-493)
Gene name: FZD6
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-36261
Expression systems:
  • Escherichia coli
  • Trichoplusia ni
  • Spodoptera frugiperda

Quick links