› 8jhi

Electron Microscopy
3.2Å resolution

FZD3-Gs complex

Released:
DOI: 10.2210/pdb8jhi/pdb
PDB entry 8jhi coloured by chain, front view.
PDB entry 8jhi coloured by chain, side view.
PDB entry 8jhi coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A framework for Frizzled-G protein coupling and implications to the PCP signaling pathways.
Zhang Z, Lin X, Wei L, Wu Y, Xu L, Wu L, Wei X, Zhao S, Zhu X, Xu F
OpenAccess logo Cell Discov 10 3 (2024)
PMID: 38182578
Related structures: EMD-36266

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-274015 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas Chain: A
Molecule details ›
Chain: A
Length: 248 amino acids
Theoretical weight: 28.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5JWF2 (Residues: 655-707, 847-1037; Coverage: 23%)
  • Best match: Q5JWF2-3 (Residues: 655-690)
Gene names: GNAS, GNAS1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 337 amino acids
Theoretical weight: 37.07 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 4-340; Coverage: 99%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: C
Molecule details ›
Chain: C
Length: 57 amino acids
Theoretical weight: 6.26 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 6-62; Coverage: 80%)
Gene name: GNG2
Sequence domains: GGL domain
Nb35 Chain: N
Molecule details ›
Chain: N
Length: 126 amino acids
Theoretical weight: 13.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Frizzled-3 Chain: R
Molecule details ›
Chain: R
Length: 520 amino acids
Theoretical weight: 60.08 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9NPG1 (Residues: 23-542; Coverage: 81%)
Gene name: FZD3
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-36266
Expression systems:
  • Escherichia coli
  • Trichoplusia ni
  • Spodoptera frugiperda

Quick links

Citations

2 review citations

Interplay between Wnt signaling molecules and exosomal miRNAs in breast cancer (Review).
Li et al. (2024)
1 more