› 8jhn

Electron Microscopy
3.75Å resolution

Structure of MMF-GPR109A-G protein complex

Released:
DOI: 10.2210/pdb8jhn/pdb
PDB entry 8jhn coloured by chain, front view.
PDB entry 8jhn coloured by chain, side view.
PDB entry 8jhn coloured by chain, top view.
Source organisms:
Primary publication:
Structure-guided engineering of biased-agonism in the human niacin receptor via single amino acid substitution.
Yadav MK, Sarma P, Maharana J, Ganguly M, Mishra S, Zaidi N, Dalal A, Singh V, Saha S, Mahajan G, Sharma S, Chami M, Banerjee R, Shukla AK
OpenAccess logo Nat Commun 15 1939 (2024)
PMID: 38431681
Related structures: EMD-36280

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-272405 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(o) subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 240 amino acids
Theoretical weight: 27.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09471 (Residues: 4-57, 183-354; Coverage: 61%)
Gene name: GNAO1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 350 amino acids
Theoretical weight: 38.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Hydroxycarboxylic acid receptor 2; Muscarinic acetylcholine receptor M4 Chain: D
Molecule details ›
Chain: D
Length: 419 amino acids
Theoretical weight: 47.8 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P08173 (Residues: 2-23, 25-26; Coverage: 5%)
  • Canonical: Q8TDS4 (Residues: 4-363; Coverage: 99%)
Gene names: CHRM4, GPR109A, HCA2, HCAR2, HM74A, NIACR1
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
ScFv16 (Antibody) Chain: H
Molecule details ›
Chain: H
Length: 248 amino acids
Theoretical weight: 26.47 KDa
Source organism: Mus musculus
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand UR9 1 x UR9
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.75Å
Relevant EMDB volumes: EMD-36280
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda

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