› 8jif

Electron Microscopy
2.28Å resolution

Cryo-EM Structure of 3-axis block of AAV9P31-Car4 complex

Released:
DOI: 10.2210/pdb8jif/pdb
PDB entry 8jif coloured by chain, front view.
PDB entry 8jif coloured by chain, side view.
PDB entry 8jif coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of the recognition of adeno-associated virus by the neurological system-related receptor carbonic anhydrase IV.
Zhang R, Liu Y, Yu F, Xu G, Li L, Li B, Lou Z
OpenAccess logo PLoS Pathog 20 e1011953 (2024)
PMID: 38315719
Related structures: EMD-36311

Function and Biology Details

Reaction catalysed: 
H(2)CO(3) = CO(2) + H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-274843 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Carbonic anhydrase 4 Chain: R
Molecule details ›
Chain: R
Length: 256 amino acids
Theoretical weight: 29.28 KDa
Source organism: Mus musculus
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q64444 (Residues: 22-277; Coverage: 89%)
Gene names: Ca4, Car4
Sequence domains: Eukaryotic-type carbonic anhydrase
Capsid protein VP1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 525 amino acids
Theoretical weight: 59.3 KDa
Source organism: Adeno-associated virus 9
Expression system: Homo sapiens
UniProt:
  • Canonical: Q6JC40 (Residues: 219-588, 589-736; Coverage: 70%)
Gene name: cap
Sequence domains: Parvovirus coat protein VP2

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.28Å
Relevant EMDB volumes: EMD-36311
Expression systems:
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Homo sapiens

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