› 8jim

Electron Microscopy
2.98Å resolution

Cryo-EM structure of MMF bound ketone body receptor HCAR2-Gi signaling complex

Released:
DOI: 10.2210/pdb8jim/pdb
PDB entry 8jim coloured by chain, front view.
PDB entry 8jim coloured by chain, side view.
PDB entry 8jim coloured by chain, top view.
Source organisms:
Primary publication:
Biased allosteric activation of ketone body receptor HCAR2 suppresses inflammation.
Zhao C, Wang H, Liu Y, Cheng L, Wang B, Tian X, Fu H, Wu C, Li Z, Shen C, Yu J, Yang S, Hu H, Fu P, Ma L, Wang C, Yan W, Shao Z
Mol Cell (2023)
PMID: 37597514
Related structures: EMD-36318

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-229975 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 356 amino acids
Theoretical weight: 39.14 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: C
Molecule details ›
Chain: C
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: D
Molecule details ›
Chain: D
Length: 354 amino acids
Theoretical weight: 40.41 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63096 (Residues: 1-354; Coverage: 100%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
scFv16 Chain: S
Molecule details ›
Chain: S
Length: 266 amino acids
Theoretical weight: 28.64 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
Hydroxycarboxylic acid receptor 2 Chain: E
Molecule details ›
Chain: E
Length: 397 amino acids
Theoretical weight: 46.03 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8TDS4 (Residues: 1-363; Coverage: 100%)
Gene names: GPR109A, HCA2, HCAR2, HM74A, NIACR1
Sequence domains: 7 transmembrane receptor (rhodopsin family)

Ligands and Environments

2 bound ligands:
Ligand AW9 1 x AW9
Ligand CLR 1 x CLR
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.98Å
Relevant EMDB volumes: EMD-36318
Expression system: Spodoptera frugiperda

Quick links

Citations

2 review citations

Structure, function and drug discovery of GPCR signaling.
Cheng et al. (2023)
1 more