8jir

Electron Microscopy
2.57Å resolution

Cryo-EM structure of the GLP-1R/GCGR dual agonist SAR425899-bound human GLP-1R-Gs complex

Released:
Primary publication:
Structural analysis of the dual agonism at GLP-1R and GCGR.
OpenAccess logo Proc Natl Acad Sci U S A 120 e2303696120 (2023)
PMID: 37549266
Related structures: EMD-36325

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-273763 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1; Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 361 amino acids
Theoretical weight: 41.89 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
SAR425899 Chain: P
Molecule details ›
Chain: P
Length: 29 amino acids
Theoretical weight: 3.24 KDa
Source organism: synthetic construct
Expression system: Not provided
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Nanobody 35 Chain: N
Glucagon-like peptide 1 receptor Chain: R

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.57Å
Relevant EMDB volumes: EMD-36325
Expression systems:
  • Spodoptera frugiperda
  • Not provided
  • Escherichia coli