› 8jj6

X-ray diffraction
2.72Å resolution

Structure of the NELF-BCE complex

Released:
DOI: 10.2210/pdb8jj6/pdb
PDB entry 8jj6 coloured by chain, front view.
PDB entry 8jj6 coloured by chain, side view.
PDB entry 8jj6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of the human negative elongation factor NELF-B/C/E ternary complex.
Cao Y, Qin Y, Zhang W, Tian W, Ren Y, Ren J, Wang J, Wang M, Jiang J, Wang Z
Biochem Biophys Res Commun 677 155-161 (2023)
PMID: 37591184

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-273290 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Negative elongation factor B Chains: A, B
Molecule details ›
Chains: A, B
Length: 560 amino acids
Theoretical weight: 63.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8WX92 (Residues: 1-560; Coverage: 97%)
Gene names: COBRA1, KIAA1182, NELFB
Sequence domains: Cofactor of BRCA1 (COBRA1)
Negative elongation factor C/D Chains: C, D
Molecule details ›
Chains: C, D
Length: 147 amino acids
Theoretical weight: 16.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8IXH7 (Residues: 36-182; Coverage: 25%)
Gene names: HSPC130, NELFCD, NELFD, TH1, TH1L
Negative elongation factor E Chains: E, F
Molecule details ›
Chains: E, F
Length: 51 amino acids
Theoretical weight: 5.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P18615 (Residues: 1-50; Coverage: 13%)
Gene names: NELFE, RD, RDBP

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P21212
Unit cell:
a: 156.614Å b: 166.709Å c: 75.025Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.206 0.262
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Distinct negative elongation factor conformations regulate RNA polymerase II promoter-proximal pausing.
Su et al. (2024)