› 8jln

Electron Microscopy
3.24Å resolution

T1AM-bound hTAAR1-Gs protein complex

Released:
DOI: 10.2210/pdb8jln/pdb
PDB entry 8jln coloured by chain, front view.
PDB entry 8jln coloured by chain, side view.
PDB entry 8jln coloured by chain, top view.
Source organisms:
Primary publication:
Ligand recognition and G protein coupling of trace amine receptor TAAR1.
Xu Z, Guo L, Yu J, Shen S, Wu C, Zhang W, Zhao C, Deng Y, Tian X, Feng Y, Hou H, Su L, Wang H, Guo S, Wang H, Wang K, Chen P, Zhao J, Zhang X, Yong X, Cheng L, Liu L, Yang S, Yang F, Wang X, Yu X, Xu Y, Sun JP, Yan W, Shao Z
Nature (2023)
PMID: 37935376
Related structures: EMD-36401

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-245755 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1; Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas; Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 371 amino acids
Theoretical weight: 43.12 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63096 (Residues: 1-5, 61-181, 248-253, 257-258, 261-263; Coverage: 39%)
  • Canonical: Q5JWF2 (Residues: 656-656, 848-913, 920-922, 925-926, 930-1037; Coverage: 17%)
  • Canonical: P63092 (Residues: 14-67; Coverage: 14%)
Gene names: GNAI1, GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 358 amino acids
Theoretical weight: 39.42 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Soluble cytochrome b562; Trace amine-associated receptor 1 Chain: R
Molecule details ›
Chain: R
Length: 470 amino acids
Theoretical weight: 54.13 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
  • Canonical: Q96RJ0 (Residues: 1-339; Coverage: 100%)
Gene names: TA1, TAAR1, TAR1, TRAR1, cybC
Sequence domains:
ScFv16 Chain: S
Molecule details ›
Chain: S
Length: 251 amino acids
Theoretical weight: 26.68 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: Y
Molecule details ›
Chain: Y
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain

Ligands and Environments

1 bound ligand:
Ligand UJF 1 x UJF
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.24Å
Relevant EMDB volumes: EMD-36401
Expression system: Spodoptera frugiperda

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