8jn1

Electron Microscopy
3.5Å resolution

Cryo-EM structure of dengue virus serotype 3 strain EHIE46200Y19 in complex with human antibody DENV-115 IgG at 4 deg C (subparticle LLR-LRR)

Released:
Entry authors: Fibriansah G, Ng TS, Tan AWK, Shi J, Lok SM
Related structures: EMD-36429

Function and Biology Details

Reactions catalysed:
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexadecamer (preferred)
PDBe Complex ID:
PDB-CPX-275698 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (6 distinct):
Envelope protein (Fragment) Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 493 amino acids
Theoretical weight: 53.73 KDa
Source organism: dengue virus type 3
Expression system: Aedes albopictus
UniProt:
  • Canonical: A0A3G1J4X7 (Residues: 281-773; Coverage: 15%)
Sequence domains:
Polyprotein Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 75 amino acids
Theoretical weight: 8.37 KDa
Source organism: dengue virus type 3
Expression system: Aedes albopictus
UniProt:
  • Canonical: A0A7D0UCF5 (Residues: 206-280; Coverage: 2%)
Gene name: POLY
Sequence domains: Flavivirus envelope glycoprotein M
Human antibody DENV-115 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 122 amino acids
Theoretical weight: 13.61 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Human antibody DENV-115 light chain Chain: L
Molecule details ›
Chain: L
Length: 110 amino acids
Theoretical weight: 11.5 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer : NEW Components: NAG, BMA
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-36429
Expression systems:
  • Aedes albopictus
  • Homo sapiens