› 8jna

X-ray diffraction
1.7Å resolution

CRAF ras-binding domain chimera, apo form

Released:
DOI: 10.2210/pdb8jna/pdb
PDB entry 8jna coloured by chain, front view.
PDB entry 8jna coloured by chain, side view.
PDB entry 8jna coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Kawamura T, Kumasaka T

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-246020 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RAF proto-oncogene serine/threonine-protein kinase; Serine/threonine-protein kinase B-raf Chain: B
Molecule details ›
Chain: B
Length: 94 amino acids
Theoretical weight: 10.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04049 (Residues: 50-98, 101-101, 115-119, 122-128, 130-141; Coverage: 11%)
  • Canonical: P15056 (Residues: 198-199, 201-210, 216-217, 225-225; Coverage: 2%)
Gene names: BRAF, BRAF1, RAF, RAF1, RAFB1
Sequence domains: Raf-like Ras-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: C2221
Unit cell:
a: 39.696Å b: 58.744Å c: 69.055Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.253 0.252 0.272
Expression system: Escherichia coli BL21(DE3)

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