› 8jow

X-ray diffraction
1.4Å resolution

Crystal structure of rabbit antibody with phosphorylated peptide bound

Released:
DOI: 10.2210/pdb8jow/pdb
PDB entry 8jow coloured by chain, front view.
PDB entry 8jow coloured by chain, side view.
PDB entry 8jow coloured by chain, top view.
Source organisms:
Entry authors: Caaveiro JMM, Kawade R, Tsumoto K

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-245810 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Antibody (scFv) Chains: A, C
Molecule details ›
Chains: A, C
Length: 246 amino acids
Theoretical weight: 25.26 KDa
Source organism: Oryctolagus cuniculus
Expression system: Escherichia coli
RAC-alpha serine/threonine-protein kinase Chains: B, D
Molecule details ›
Chains: B, D
Length: 12 amino acids
Theoretical weight: 1.51 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P31749 (Residues: 466-477; Coverage: 3%)
Gene names: AKT1, PKB, RAC

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
1 modified residue:
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P1
Unit cell:
a: 43.52Å b: 43.56Å c: 63.52Å
α: 71.31° β: 84.39° γ: 87.57°
R-values:
R R work R free
0.163 0.162 0.199
Expression systems:
  • Escherichia coli
  • Not provided

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