› 8jpc

Electron Microscopy
3.07Å resolution

cryo-EM structure of NTSR1-GRK2-Galpha(q) complexes 2

Released:
DOI: 10.2210/pdb8jpc/pdb
PDB entry 8jpc coloured by chain, front view.
PDB entry 8jpc coloured by chain, side view.
PDB entry 8jpc coloured by chain, top view.
Source organisms:
Primary publication:
GPCR activation and GRK2 assembly by a biased intracellular agonist.
Duan J, Liu H, Zhao F, Yuan Q, Ji Y, Cai X, He X, Li X, Li J, Wu K, Gao T, Zhu S, Lin S, Wang MW, Cheng X, Yin W, Jiang Y, Yang D, Xu HE
Nature (2023)
PMID: 37532940
Related structures: EMD-36475

Function and Biology Details

Reaction catalysed: 
ATP + [beta-adrenergic receptor] = ADP + [beta-adrenergic receptor] phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-273348 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
NTS(8-13) Chain: L
Molecule details ›
Chain: L
Length: 6 amino acids
Theoretical weight: 819 Da
Source organism: synthetic construct
Expression system: Not provided
Neurotensin receptor type 1 Chain: R
Molecule details ›
Chain: R
Length: 418 amino acids
Theoretical weight: 46.31 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P30989 (Residues: 1-418; Coverage: 100%)
Gene names: NTRR, NTSR1
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Beta-adrenergic receptor kinase 1 Chain: G
Molecule details ›
Chain: G
Length: 688 amino acids
Theoretical weight: 79.64 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P21146 (Residues: 2-689; Coverage: 100%)
Gene names: ADRBK1, GRK2
Sequence domains:
Guanine nucleotide-binding protein G(q) subunit alpha Chain: Q
Molecule details ›
Chain: Q
Length: 353 amino acids
Theoretical weight: 41.28 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P50148 (Residues: 37-359; Coverage: 90%)
Gene names: GAQ, GNAQ
Sequence domains: G-protein alpha subunit

Ligands and Environments

5 bound ligands:
Ligand SRW 1 x SRW
Ligand STU 1 x STU
Ligand GDP 1 x GDP
Ligand MG 1 x MG
Ligand ALF 1 x ALF
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.07Å
Relevant EMDB volumes: EMD-36475
Expression systems:
  • Not provided
  • Spodoptera frugiperda

Quick links

Citations

7 review citations

G protein-coupled receptors (GPCRs): advances in structures, mechanisms, and drug discovery.
Zhang et al. (2024)
6 more