› 8jpv

X-ray diffraction
2.51Å resolution

Glutamyl-tRNA synthetase from Methylacidiphilum fumariolicum

Released:
DOI: 10.2210/pdb8jpv/pdb
PDB entry 8jpv coloured by chain, front view.
PDB entry 8jpv coloured by chain, side view.
PDB entry 8jpv coloured by chain, top view.
Source organism: Methylacidiphilum fumariolicum
Entry authors: Dev A, Basu G

Function and Biology Details

Reaction catalysed: 
ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-271298 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 448 amino acids
Theoretical weight: 51.79 KDa
Source organism: Methylacidiphilum fumariolicum
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: I0JVL9 (Residues: 1-448; Coverage: 100%)
Gene names: MFUM_1020011, gltX
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU R-AXIS IV
Spacegroup: P212121
Unit cell:
a: 40.31Å b: 62.54Å c: 190.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.209 0.266
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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