› 8jro

Electron Microscopy
3.01Å resolution

Structure of E6AP-E6 complex in Att2 state

Released:
DOI: 10.2210/pdb8jro/pdb
PDB entry 8jro coloured by chain, front view.
PDB entry 8jro coloured by chain, side view.
PDB entry 8jro coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into the functional mechanism of the ubiquitin ligase E6AP.
Wang Z, Fan F, Li Z, Ye F, Wang Q, Gao R, Qiu J, Lv Y, Lin M, Xu W, Luo C, Yu X
OpenAccess logo Nat Commun 15 3531 (2024)
PMID: 38670961
Related structures: EMD-36600

Function and Biology Details

Reaction catalysed: 
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-245049 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein E6 Chains: B, D
Molecule details ›
Chains: B, D
Length: 158 amino acids
Theoretical weight: 19.16 KDa
Source organism: Human papillomavirus 16
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P03126 (Residues: 1-158; Coverage: 100%)
Gene name: E6
Sequence domains: Early Protein (E6)
Ubiquitin-protein ligase E3A Chains: A, C
Molecule details ›
Chains: A, C
Length: 875 amino acids
Theoretical weight: 100.78 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q05086 (Residues: 1-875; Coverage: 100%)
Gene names: E6AP, EPVE6AP, HPVE6A, UBE3A
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.01Å
Relevant EMDB volumes: EMD-36600
Expression system: Spodoptera frugiperda

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