› 8js7

X-ray diffraction
2.85Å resolution

Dimeric PAS domains of oxygen sensor FixL in complex with imidazole-bound heme

Released:
DOI: 10.2210/pdb8js7/pdb
PDB entry 8js7 coloured by chain, front view.
PDB entry 8js7 coloured by chain, side view.
PDB entry 8js7 coloured by chain, top view.
Source organism: Bradyrhizobium diazoefficiens USDA 110
Entry authors: Kamaya M, Koteishi H, Sawai H, Sugimoto H, Shiro Y

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149895 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sensor protein FixL Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 273 amino acids
Theoretical weight: 30.66 KDa
Source organism: Bradyrhizobium diazoefficiens USDA 110
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P23222 (Residues: 2-274; Coverage: 54%)
Gene names: bll2760, fixL
Sequence domains: PAS fold

Ligands and Environments


Cofactor: Ligand HEM 10 x HEM
2 bound ligands:
Ligand GOL 15 x GOL
Ligand IMD 10 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: C2221
Unit cell:
a: 188.854Å b: 199.313Å c: 270.01Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.216 0.248
Expression system: Escherichia coli BL21(DE3)

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