› 8jsg

Electron Microscopy
4.6Å resolution

Structure of the 30S-IF3 complex from Escherichia coli

Released:
DOI: 10.2210/pdb8jsg/pdb
PDB entry 8jsg coloured by chain, front view.
PDB entry 8jsg coloured by chain, side view.
PDB entry 8jsg coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Initiation factor 3 bound to the 30S ribosomal subunit in an initial step of translation.
Uday AB, Mishra RK, Hussain T
Proteins (2023)
PMID: 38148682
Related structures: EMD-36619

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
hetero 22-mer (preferred)
PDBe Complex ID:
PDB-CPX-272115 (preferred)
Entry contents:
21 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (22 distinct):
Small ribosomal subunit protein bS18 Chain: 1
Molecule details ›
Chain: 1
Length: 73 amino acids
Theoretical weight: 8.75 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7T7 (Residues: 2-74; Coverage: 97%)
Gene names: JW4160, b4202, rpsR
Sequence domains: Ribosomal protein S18
Small ribosomal subunit protein bS21 Chain: 2
Molecule details ›
Chain: 2
Length: 53 amino acids
Theoretical weight: 6.26 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P68679 (Residues: 2-54; Coverage: 75%)
Gene names: JW3037, b3065, rpsU
Sequence domains: Ribosomal protein S21
Small ribosomal subunit protein bS20 Chain: 3
Molecule details ›
Chain: 3
Length: 86 amino acids
Theoretical weight: 9.58 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7U7 (Residues: 2-87; Coverage: 99%)
Gene names: JW0022, b0023, rpsT
Sequence domains: Ribosomal protein S20
Translation initiation factor IF-3 Chain: A
Molecule details ›
Chain: A
Length: 180 amino acids
Theoretical weight: 20.6 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A707 (Residues: 1-180; Coverage: 100%)
Gene names: JW5829, b1718, fit, infC, srjA
Sequence domains:
Small ribosomal subunit protein uS17 Chain: P
Molecule details ›
Chain: P
Length: 82 amino acids
Theoretical weight: 9.48 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG63 (Residues: 2-83; Coverage: 98%)
Gene names: JW3273, b3311, neaA, rpsQ
Sequence domains: Ribosomal protein S17
Small ribosomal subunit protein uS5 Chain: k
Molecule details ›
Chain: k
Length: 158 amino acids
Theoretical weight: 16.64 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7W1 (Residues: 2-159; Coverage: 95%)
Gene names: JW3265, b3303, rpsE, spc
Sequence domains:
Small ribosomal subunit protein uS4 Chain: l
Molecule details ›
Chain: l
Length: 205 amino acids
Theoretical weight: 23.38 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7V8 (Residues: 2-206; Coverage: 100%)
Gene names: JW3258, b3296, ramA, rpsD
Sequence domains:
Small ribosomal subunit protein bS6, non-modified isoform Chain: n
Molecule details ›
Chain: n
Length: 100 amino acids
Theoretical weight: 11.67 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P02358 (Residues: 1-100; Coverage: 74%)
Gene names: JW4158, b4200, rpsF
Sequence domains: Ribosomal protein S6
Small ribosomal subunit protein uS8 Chain: p
Molecule details ›
Chain: p
Length: 129 amino acids
Theoretical weight: 14.02 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7W7 (Residues: 2-130; Coverage: 99%)
Gene names: JW3268, b3306, rpsH
Sequence domains: Ribosomal protein S8
Small ribosomal subunit protein uS11 Chain: q
Molecule details ›
Chain: q
Length: 128 amino acids
Theoretical weight: 13.74 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7R9 (Residues: 2-129; Coverage: 99%)
Gene names: JW3259, b3297, rpsK
Sequence domains: Ribosomal protein S11
Small ribosomal subunit protein uS12 Chain: t
Molecule details ›
Chain: t
Length: 123 amino acids
Theoretical weight: 13.64 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7S3 (Residues: 2-124; Coverage: 99%)
Gene names: JW3304, b3342, rpsL, strA
Sequence domains: Ribosomal protein S12/S23
Small ribosomal subunit protein uS15 Chain: u
Molecule details ›
Chain: u
Length: 88 amino acids
Theoretical weight: 10.16 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0ADZ4 (Residues: 2-89; Coverage: 99%)
Gene names: JW3134, b3165, rpsO, secC
Sequence domains: Ribosomal protein S15
Small ribosomal subunit protein bS16 Chain: y
Molecule details ›
Chain: y
Length: 82 amino acids
Theoretical weight: 9.21 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7T3 (Residues: 1-82; Coverage: 100%)
Gene names: JW2590, b2609, rpsP
Sequence domains: Ribosomal protein S16
Small ribosomal subunit protein uS3 Chain: h
Molecule details ›
Chain: h
Length: 206 amino acids
Theoretical weight: 23.08 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7V3 (Residues: 2-207; Coverage: 88%)
Gene names: JW3276, b3314, rpsC
Sequence domains:
Small ribosomal subunit protein uS7 Chain: m
Molecule details ›
Chain: m
Length: 151 amino acids
Theoretical weight: 16.86 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P02359 (Residues: 2-152; Coverage: 84%)
Gene names: JW3303, b3341, rpsG
Sequence domains: Ribosomal protein S7p/S5e
Small ribosomal subunit protein uS9 Chain: o
Molecule details ›
Chain: o
Length: 129 amino acids
Theoretical weight: 14.76 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7X3 (Residues: 2-130; Coverage: 99%)
Gene names: JW3199, b3230, rpsI
Sequence domains: Ribosomal protein S9/S16
Small ribosomal subunit protein uS10 Chain: r
Molecule details ›
Chain: r
Length: 102 amino acids
Theoretical weight: 11.7 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7R5 (Residues: 1-102; Coverage: 99%)
Gene names: JW3283, b3321, nusE, rpsJ
Sequence domains: Ribosomal protein S10p/S20e
Small ribosomal subunit protein uS13 Chain: s
Molecule details ›
Chain: s
Length: 114 amino acids
Theoretical weight: 12.63 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7S9 (Residues: 2-115; Coverage: 97%)
Gene names: JW3260, b3298, rpsM
Sequence domains: Ribosomal protein S13/S18
Small ribosomal subunit protein uS14 Chain: w
Molecule details ›
Chain: w
Length: 100 amino acids
Theoretical weight: 11.48 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG59 (Residues: 2-101; Coverage: 99%)
Gene names: JW3269, b3307, rpsN
Sequence domains: Ribosomal protein S14p/S29e
Small ribosomal subunit protein uS19 Chain: z
Molecule details ›
Chain: z
Length: 80 amino acids
Theoretical weight: 9.15 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7U3 (Residues: 2-81; Coverage: 87%)
Gene names: JW3278, b3316, rpsS
Sequence domains: Ribosomal protein S19
Small ribosomal subunit protein uS2 Chain: j
Molecule details ›
Chain: j
Length: 225 amino acids
Theoretical weight: 25.02 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7V0 (Residues: 2-226; Coverage: 93%)
Gene names: JW0164, b0169, rpsB
Sequence domains: Ribosomal protein S2
16S ribosomal RNA Chain: g
Molecule details ›
Chain: g
Length: 1540 nucleotides
Theoretical weight: 499.08 KDa
Sequence domains: Bacterial small subunit ribosomal RNA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.6Å
Relevant EMDB volumes: EMD-36619
Expression system: Escherichia coli BL21(DE3)

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