› 8jsh

Electron Microscopy
4.4Å resolution

Structure of the 30S-body-IF3 complex from Escherichia coli

Released:
DOI: 10.2210/pdb8jsh/pdb
PDB entry 8jsh coloured by chain, front view.
PDB entry 8jsh coloured by chain, side view.
PDB entry 8jsh coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Initiation factor 3 bound to the 30S ribosomal subunit in an initial step of translation.
Uday AB, Mishra RK, Hussain T
Proteins (2023)
PMID: 38148682
Related structures: EMD-36620

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
PDBe Complex ID:
PDB-CPX-239068 (preferred)
Entry contents:
13 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (14 distinct):
Small ribosomal subunit protein bS18 Chain: 1
Molecule details ›
Chain: 1
Length: 75 amino acids
Theoretical weight: 9.01 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7T7 (Residues: 1-75; Coverage: 100%)
Gene names: JW4160, b4202, rpsR
Sequence domains: Ribosomal protein S18
Small ribosomal subunit protein bS21 Chain: 2
Molecule details ›
Chain: 2
Length: 71 amino acids
Theoretical weight: 8.52 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P68679 (Residues: 1-71; Coverage: 100%)
Gene names: JW3037, b3065, rpsU
Sequence domains: Ribosomal protein S21
Small ribosomal subunit protein bS20 Chain: 3
Molecule details ›
Chain: 3
Length: 87 amino acids
Theoretical weight: 9.71 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7U7 (Residues: 1-87; Coverage: 100%)
Gene names: JW0022, b0023, rpsT
Sequence domains: Ribosomal protein S20
Small ribosomal subunit protein uS17 Chain: P
Molecule details ›
Chain: P
Length: 84 amino acids
Theoretical weight: 9.72 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG63 (Residues: 1-84; Coverage: 100%)
Gene names: JW3273, b3311, neaA, rpsQ
Sequence domains: Ribosomal protein S17
Small ribosomal subunit protein uS5 Chain: k
Molecule details ›
Chain: k
Length: 167 amino acids
Theoretical weight: 17.63 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7W1 (Residues: 1-167; Coverage: 100%)
Gene names: JW3265, b3303, rpsE, spc
Sequence domains:
Small ribosomal subunit protein uS4 Chain: l
Molecule details ›
Chain: l
Length: 206 amino acids
Theoretical weight: 23.51 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7V8 (Residues: 1-206; Coverage: 100%)
Gene names: JW3258, b3296, ramA, rpsD
Sequence domains:
Small ribosomal subunit protein bS6 Chain: n
Molecule details ›
Chain: n
Length: 135 amino acids
Theoretical weight: 15.73 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P02358 (Residues: 1-135; Coverage: 100%)
Gene names: JW4158, b4200, rpsF
Sequence domains: Ribosomal protein S6
Small ribosomal subunit protein uS8 Chain: p
Molecule details ›
Chain: p
Length: 130 amino acids
Theoretical weight: 14.15 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7W7 (Residues: 1-130; Coverage: 100%)
Gene names: JW3268, b3306, rpsH
Sequence domains: Ribosomal protein S8
Small ribosomal subunit protein uS11 Chain: q
Molecule details ›
Chain: q
Length: 129 amino acids
Theoretical weight: 13.87 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7R9 (Residues: 1-129; Coverage: 100%)
Gene names: JW3259, b3297, rpsK
Sequence domains: Ribosomal protein S11
Small ribosomal subunit protein uS12 Chain: t
Molecule details ›
Chain: t
Length: 124 amino acids
Theoretical weight: 13.77 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7S3 (Residues: 1-124; Coverage: 100%)
Gene names: JW3304, b3342, rpsL, strA
Sequence domains: Ribosomal protein S12/S23
Small ribosomal subunit protein uS15 Chain: u
Molecule details ›
Chain: u
Length: 89 amino acids
Theoretical weight: 10.29 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0ADZ4 (Residues: 1-89; Coverage: 100%)
Gene names: JW3134, b3165, rpsO, secC
Sequence domains: Ribosomal protein S15
Small ribosomal subunit protein bS16 Chain: y
Molecule details ›
Chain: y
Length: 82 amino acids
Theoretical weight: 9.21 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7T3 (Residues: 1-82; Coverage: 100%)
Gene names: JW2590, b2609, rpsP
Sequence domains: Ribosomal protein S16
Translation initiation factor IF-3 Chain: A
Molecule details ›
Chain: A
Length: 180 amino acids
Theoretical weight: 20.6 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C3T7P7 (Residues: 1-180; Coverage: 100%)
Gene names: A5U30_001025, A8502_003612, ACN81_10665, ACU57_21950, AW118_00780, AWP47_09575, B6R15_001939, B6R31_000296, BCB93_002840, BE932_04040, BER14_00770, BG944_000165, BGM66_002496, BJI68_18100, BK292_18190, BK383_05675, BKL28_002577, BR158_000328, BTB68_001582, BTQ06_20635, BXT93_24060, C0P57_002913, C1Q91_000515, C2121_000650, C2M16_14700, C2R31_000196, C3F40_02120, C9Z68_00820, CF22_000721, CG704_14170, CIG67_20525, CQ842_05635, CQ842_22885, CTR35_003950, D3C88_17245, D3G36_00505, D4M65_05395, D4N09_09285, D9D43_00200, D9E49_08595, D9J61_01950, DD762_27015, DIV22_02140, DNX30_01350, DS732_14305, DTL43_16720, DU321_09090, E4K51_04355, E5H86_01650, E6D34_01840, EAI46_22595, ECs2425, EIA08_08045, EIZ93_02200, EN85_001570, EPS76_32375, EPS97_03970, EWK56_01325, F7F11_07955, F7N46_05260, F9413_02060, F9461_08590, F9B07_05800, FIJ20_00895, FJQ40_22235, FKO60_09985, FOI11_004460, FOI11_15585, FPI65_10560, FPS11_12925, FVB16_15620, FWK02_19185, FZU14_13205, G3V95_02020, G3W53_07400, G4A38_00735, G4A47_06090, G5603_11480, GAI89_08015, GAJ12_14820, GFY48_09965, GKF66_01295, GNW61_13600, GNZ05_11155, GOP25_01020, GP711_11870, GP965_16285, GP975_05605, GQA06_10390, GQE86_12395, GQM04_13825, GQM13_04975, GQM21_17650, GQN34_01510, GQW07_05880, GRC73_09620, GRO95_04065, GRW05_00690, GRW24_08140, GUC01_03630, H0O53_16545, H0O72_06350, HEP30_001085, HEP34_002140, HHH44_000857, HI055_000501, HIE29_000939, HJQ60_004740, HLX92_05390, HLZ50_03355, HMV95_01060, HMW38_05035, HV109_10365, HV209_08105, HVV39_26030, HVW04_02085, HVW43_02400, HVY77_11835, I6H02_02040, J0541_000497, J5U05_000737, JNP96_15170, OGM49_12935, P6223_003444, QDW62_11910, RZR61_20400, WR15_13835, infC
Sequence domains:
16S ribosomal RNA Chain: g
Molecule details ›
Chain: g
Length: 1539 nucleotides
Theoretical weight: 498.73 KDa
Sequence domains: Bacterial small subunit ribosomal RNA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.4Å
Relevant EMDB volumes: EMD-36620
Expression system: Escherichia coli BL21(DE3)

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