› 8jsp

Electron Microscopy
3.65Å resolution

Ulotaront(SEP-363856)-bound Serotonin 1A (5-HT1A) receptor-Gi complex

Released:
DOI: 10.2210/pdb8jsp/pdb
PDB entry 8jsp coloured by chain, front view.
PDB entry 8jsp coloured by chain, side view.
PDB entry 8jsp coloured by chain, top view.
Source organisms:
Primary publication:
Ligand recognition and G protein coupling of trace amine receptor TAAR1.
Xu Z, Guo L, Yu J, Shen S, Wu C, Zhang W, Zhao C, Deng Y, Tian X, Feng Y, Hou H, Su L, Wang H, Guo S, Wang H, Wang K, Chen P, Zhao J, Zhang X, Yong X, Cheng L, Liu L, Yang S, Yang F, Wang X, Yu X, Xu Y, Sun JP, Yan W, Shao Z
Nature (2023)
PMID: 37935376
Related structures: EMD-36626

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-235763 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 351 amino acids
Theoretical weight: 40.12 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63096 (Residues: 4-354; Coverage: 99%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 328 amino acids
Theoretical weight: 35.99 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 13-340; Coverage: 97%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
ScFv16 Chain: E
Molecule details ›
Chain: E
Length: 250 amino acids
Theoretical weight: 26.59 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 45 amino acids
Theoretical weight: 4.99 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 18-62; Coverage: 63%)
Gene name: GNG2
Sequence domains: GGL domain
5-hydroxytryptamine receptor 1A Chain: R
Molecule details ›
Chain: R
Length: 381 amino acids
Theoretical weight: 42 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P08908 (Residues: 35-415; Coverage: 90%)
Gene names: ADRB2RL1, ADRBRL1, HTR1A
Sequence domains: 7 transmembrane receptor (rhodopsin family)

Ligands and Environments

1 bound ligand:
Ligand UJL 1 x UJL
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.65Å
Relevant EMDB volumes: EMD-36626
Expression system: Spodoptera frugiperda

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