› 8juu

Electron Microscopy
3.8Å resolution

rat megalin

Released:
DOI: 10.2210/pdb8juu/pdb
PDB entry 8juu coloured by chain, front view.
PDB entry 8juu coloured by chain, side view.
PDB entry 8juu coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Cryo-EM structures elucidate the multiligand receptor nature of megalin.
Goto S, Tsutsumi A, Lee Y, Hosojima M, Kabasawa H, Komochi K, Nagatoishi S, Takemoto K, Tsumoto K, Nishizawa T, Kikkawa M, Saito A
Proc Natl Acad Sci U S A 121 e2318859121 (2024)
PMID: 38771880
Related structures: EMD-36664

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-247404 (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (11 distinct):
Low-density lipoprotein receptor-related protein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 4660 amino acids
Theoretical weight: 519.87 KDa
Source organism: Rattus norvegicus
UniProt:
  • Canonical: P98158 (Residues: 1-4660; Coverage: 100%)
Gene name: Lrp2
Sequence domains:
unclear peptide Chains: C, I
Molecule details ›
Chains: C, I
Length: 6 amino acids
Theoretical weight: 557 Da
Source organism: Rattus norvegicus
unclear peptide Chains: D, J
Molecule details ›
Chains: D, J
Length: 3 amino acids
Theoretical weight: 291 Da
Source organism: Rattus norvegicus
unclear peptide Chains: G, K
Molecule details ›
Chains: G, K
Length: 5 amino acids
Theoretical weight: 532 Da
Source organism: Rattus norvegicus
unclear peptide Chains: H, L, O, R
Molecule details ›
Chains: H, L, O, R
Length: 5 amino acids
Theoretical weight: 473 Da
Source organism: Rattus norvegicus
unclear peptide Chains: M, P
Molecule details ›
Chains: M, P
Length: 6 amino acids
Theoretical weight: 529 Da
Source organism: Rattus norvegicus
unclear peptide Chains: N, Q
Molecule details ›
Chains: N, Q
Length: 5 amino acids
Theoretical weight: 473 Da
Source organism: Rattus norvegicus

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
4 bound ligands:
Ligand NAG 24 x NAG
Ligand A2G 22 x A2G
Ligand CA 88 x CA
Ligand NI 2 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-36664

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