8jx2

Electron Microscopy
2.2Å resolution

alpha-Hemolysin(G122S/K147R)-SpyTag/SpyCatcher head to head 14-mer

Released:
Source organism: Staphylococcus aureus
Entry authors: Ishii Y, Naito K, Yokoyama T, Tanaka Y, Matsuura T
Related structures: EMD-36688

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-272495 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Alpha-hemolysin Chains: A, B, C, D, E, F, G
Molecule details ›
Chains: A, B, C, D, E, F, G
Length: 421 amino acids
Theoretical weight: 47 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P09616 (Residues: 27-319; Coverage: 100%)
Gene names: hla, hly
Sequence domains: Leukocidin/Hemolysin toxin family
Alpha-hemolysin Chains: H, I, J, K, L, M, N
Molecule details ›
Chains: H, I, J, K, L, M, N
Length: 324 amino acids
Theoretical weight: 36.8 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P09616 (Residues: 27-319; Coverage: 100%)
Gene names: hla, hly
Sequence domains: Leukocidin/Hemolysin toxin family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.2Å
Relevant EMDB volumes: EMD-36688
Expression system: Escherichia coli