› 8jxh

Electron Microscopy
3.5Å resolution

rat megalin RAP complex wingA

Released:
DOI: 10.2210/pdb8jxh/pdb
PDB entry 8jxh coloured by chain, front view.
PDB entry 8jxh coloured by chain, side view.
PDB entry 8jxh coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Cryo-EM structures elucidate the multiligand receptor nature of megalin.
Goto S, Tsutsumi A, Lee Y, Hosojima M, Kabasawa H, Komochi K, Nagatoishi S, Takemoto K, Tsumoto K, Nishizawa T, Kikkawa M, Saito A
Proc Natl Acad Sci U S A 121 e2318859121 (2024)
PMID: 38771880
Related structures: EMD-36701

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-245918 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (7 distinct):
Alpha-2-macroglobulin receptor-associated protein Chain: D
Molecule details ›
Chain: D
Length: 332 amino acids
Theoretical weight: 38.91 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99068 (Residues: 34-360; Coverage: 100%)
Gene name: Lrpap1
Sequence domains:
Low-density lipoprotein receptor-related protein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 4660 amino acids
Theoretical weight: 519.87 KDa
Source organism: Rattus norvegicus
UniProt:
  • Canonical: P98158 (Residues: 1-4660; Coverage: 100%)
Gene name: Lrp2
Sequence domains:
unclear peptide Chain: Q
Molecule details ›
Chain: Q
Length: 6 amino acids
Theoretical weight: 529 Da
Source organism: Rattus norvegicus
unclear peptide Chain: R
Molecule details ›
Chain: R
Length: 5 amino acids
Theoretical weight: 473 Da
Source organism: Rattus norvegicus

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
3 bound ligands:
Ligand NAG 4 x NAG
Ligand A2G 9 x A2G
Ligand CA 16 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-36701
Expression system: Escherichia coli

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