› 8jy0

X-ray diffraction
2.75Å resolution

Crystal structure of RhoBAST complexed with TMR-DN

Released:
DOI: 10.2210/pdb8jy0/pdb
PDB entry 8jy0 coloured by chain, front view.
PDB entry 8jy0 coloured by chain, side view.
PDB entry 8jy0 coloured by chain, top view.
Source organisms:
Primary publication:
Structural mechanisms for binding and activation of a contact-quenched fluorophore by RhoBAST.
Zhang Y, Xu Z, Xiao Y, Jiang H, Zuo X, Li X, Fang X
Nat Commun 15 4206 (2024)
PMID: 38760339

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-246912 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
U1 small nuclear ribonucleoprotein A Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 102 amino acids
Theoretical weight: 11.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09012 (Residues: 1-102; Coverage: 36%)
Gene name: SNRPA
Sequence domains: RNA recognition motif
RhoBAST Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 64 nucleotides
Theoretical weight: 20.61 KDa
Source organism: artificial sequences
Expression system: in vitro transcription vector pT7-TP(deltai)

Ligands and Environments

5 bound ligands:
Ligand MG 4 x MG
Ligand GDP 3 x GDP
Ligand PGE 1 x PGE
Ligand V8C 3 x V8C
Ligand V7U 1 x V7U
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: I212121
Unit cell:
a: 108.531Å b: 158.936Å c: 182.424Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.202 0.231
Expression systems:
  • Escherichia coli BL21(DE3)
  • in vitro transcription vector pT7-TP(deltai)

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