› 8jz1

X-ray diffraction
1.24Å resolution

Crystal structure of a single-chain monellin mutant C41V

Released:
DOI: 10.2210/pdb8jz1/pdb
PDB entry 8jz1 coloured by chain, front view.
PDB entry 8jz1 coloured by chain, side view.
PDB entry 8jz1 coloured by chain, top view.
Source organism: Dioscoreophyllum cumminsii
Primary publication:
Investigating the Effect of Substituting a Single Cysteine Residue on the Thermal Stability of an Engineered Sweet Protein, Single-Chain Monellin.
Ohnuma K, Yamashita A, Yasui N
OpenAccess logo Protein J (2023)
PMID: 37737932

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136325 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Monellin chain A; Monellin chain B Chains: A, B
Molecule details ›
Chains: A, B
Length: 96 amino acids
Theoretical weight: 11.22 KDa
Source organism: Dioscoreophyllum cumminsii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02882 (Residues: 1-48; Coverage: 96%)
  • Canonical: P02881 (Residues: 2-45; Coverage: 98%)
Sequence domains: Monellin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 39.115Å b: 48.685Å c: 47.162Å
α: 90° β: 102.66° γ: 90°
R-values:
R R work R free
0.168 0.166 0.2
Expression system: Escherichia coli BL21(DE3)

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