› 8jz4

X-ray diffraction
2.08Å resolution

Crystal structure of AetF in complex with FAD and 5-bromo-L-tryptophan

Released:
DOI: 10.2210/pdb8jz4/pdb
PDB entry 8jz4 coloured by chain, front view.
PDB entry 8jz4 coloured by chain, side view.
PDB entry 8jz4 coloured by chain, top view.
Source organism: Aetokthonos hydrillicola Thurmond2011
Primary publication:
Structural and functional insights into the self-sufficient flavin-dependent halogenase.
Dai L, Li H, Dai S, Zhang Q, Zheng H, Hu Y, Guo RT, Chen CC
Int J Biol Macromol 260 129312 (2024)
PMID: 38216020

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-217947 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AetF Chain: A
Molecule details ›
Chain: A
Length: 679 amino acids
Theoretical weight: 78.56 KDa
Source organism: Aetokthonos hydrillicola Thurmond2011
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A861B9Z9 (Residues: 1-668; Coverage: 100%)
Gene name: aetF

Ligands and Environments

2 bound ligands:
Ligand FAD 1 x FAD
Ligand 64X 1 x 64X
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE TPS 05A
Spacegroup: P212121
Unit cell:
a: 58.608Å b: 75.937Å c: 144.239Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.19 0.261
Expression system: Escherichia coli BL21(DE3)

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