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Electron Microscopy
2.86Å resolution

CryoEM structure of the NADP-dependent malic enzyme MaeB

Released:
Source organism: Escherichia coli K-12
Entry authors: Jiang WX, Cheng XQ, Wu M, Ma LX, Xing Q
Related structures: EMD-36749

Function and Biology Details

Reaction catalysed:
(S)-malate + NADP(+) = pyruvate + CO(2) + NADPH
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
PDBe Complex ID:
PDB-CPX-274166 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADP-dependent malic enzyme Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 759 amino acids
Theoretical weight: 82.51 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P76558 (Residues: 1-759; Coverage: 100%)
Gene names: JW2447, b2463, maeB, ypfF
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.86Å
Relevant EMDB volumes: EMD-36749
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'