› 8k0s

Electron Microscopy
3.41Å resolution

The Anoxybacillus pushchinoensis ORF-less Group IIC Intron HYER1 with 10-nt TRS at symmetric apo state

Released:
DOI: 10.2210/pdb8k0s/pdb
PDB entry 8k0s coloured by chain, front view.
PDB entry 8k0s coloured by chain, side view.
PDB entry 8k0s coloured by chain, top view.
Source organism: Anoxybacillus pushchinoensis
Primary publication:
Hydrolytic endonucleolytic ribozyme (HYER) is programmable for sequence-specific DNA cleavage.
Liu ZX, Zhang S, Zhu HZ, Chen ZH, Yang Y, Li LQ, Lei Y, Liu Y, Li DY, Sun A, Li CP, Tan SQ, Wang GL, Shen JY, Jin S, Gao C, Liu JG
Science 383 eadh4859 (2024)
PMID: 38301022
Related structures: EMD-36779

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-244457 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
RNA (543-MER) Chains: A, C
Molecule details ›
Chains: A, C
Length: 637 nucleotides
Theoretical weight: 206.4 KDa
Source organism: Anoxybacillus pushchinoensis
Expression system: Escherichia coli
Sequence domains:
  • Group II catalytic intron D1-D4-3
  • Group II catalytic intron D1-D4-1
  • Group II catalytic intron

    • Ligands and Environments

    1 bound ligand:
    Ligand MG 2 x MG
    No modified residues

    Experiments and Validation Details

    Entry percentile scores
    Resolution: 3.41Å
    Relevant EMDB volumes: EMD-36779
    Expression system: Escherichia coli

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