› 8k1h

X-ray diffraction
2.1Å resolution

Crystal structure of ethylene glycol-bound glycerol dehydrogenase from Klebsiella pneumoniae

Released:
DOI: 10.2210/pdb8k1h/pdb
PDB entry 8k1h coloured by chain, front view.
PDB entry 8k1h coloured by chain, side view.
PDB entry 8k1h coloured by chain, top view.
Source organism: Klebsiella pneumoniae
Primary publication:
Crystal Structure of Glycerol Dehydrogenase from Klebsiella pneumoniae
Ko GS, Nguyen TQ, Kho S, Kang W
J. Korean Chem. Soc. 68 32-39 (2024)

Function and Biology Details

Reaction catalysed: 
Glycerol + NAD(+) = glycerone + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-244729 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycerol dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 367 amino acids
Theoretical weight: 38.78 KDa
Source organism: Klebsiella pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: A6TGD6 (Residues: 1-367; Coverage: 100%)
Gene names: KPN_04241, gldA
Sequence domains: Iron-containing alcohol dehydrogenase

Ligands and Environments


Cofactor: Ligand NAD 1 x NAD
3 bound ligands:
Ligand ZN 1 x ZN
Ligand EDO 2 x EDO
Ligand TLA 2 x TLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 11C
Spacegroup: I422
Unit cell:
a: 179.732Å b: 179.732Å c: 84.923Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.17 0.192
Expression system: Escherichia coli

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