› 8k1s

Electron Microscopy
2.83Å resolution

Potassium transporter KtrAB from Bacillus subtilis in ADP-bound state

Released:
DOI: 10.2210/pdb8k1s/pdb
PDB entry 8k1s coloured by chain, front view.
PDB entry 8k1s coloured by chain, side view.
PDB entry 8k1s coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Structural basis and synergism of ATP and Na+ activation in bacterial K+ uptake system KtrAB.
Chiang WT, Chang YK, Hui WH, Chang SW, Liao CY, Chang YC, Chen CJ, Wang WC, Lai CC, Wang CH, Luo SY, Huang YP, Chou SH, Horng TL, Hou MH, Muench SP, Chen RS, Tsai MD, Hu NJ
OpenAccess logo Nat Commun 15 3850 (2024)
PMID: 38719864
Related structures: EMD-36800

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128411 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ktr system potassium uptake protein A Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 222 amino acids
Theoretical weight: 24.92 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: O32080 (Residues: 1-222; Coverage: 100%)
Gene names: BSU31090, ktrA, yuaA
Sequence domains:
Ktr system potassium uptake protein B Chains: I, J, K, L
Molecule details ›
Chains: I, J, K, L
Length: 445 amino acids
Theoretical weight: 48.47 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: O32081 (Residues: 1-445; Coverage: 100%)
Gene names: BSU31100, ktrB, yubG
Sequence domains: Cation transport protein

Ligands and Environments

2 bound ligands:
Ligand ADP 8 x ADP
Ligand K 4 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.83Å
Relevant EMDB volumes: EMD-36800
Expression system: Escherichia coli

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