8k28

Electron Microscopy
3.54Å resolution

ICP1 Csy-dsDNA complex (form 1)

Released:
Source organism: Vibrio phage ICP1_2004_A
Entry authors: Zhang LX, Feng Y
Related structures: EMD-36831

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-270573 (preferred)
Entry contents:
4 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (7 distinct):
Csy1 Chain: A
Molecule details ›
Chain: A
Length: 179 amino acids
Theoretical weight: 20.41 KDa
Source organism: Vibrio phage ICP1_2004_A
Expression system: Escherichia coli
UniProt:
  • Canonical: F1D5V8 (Residues: 1-179; Coverage: 100%)
Gene name: TUST1-10_00450
Csy2 Chain: B
Molecule details ›
Chain: B
Length: 248 amino acids
Theoretical weight: 27.4 KDa
Source organism: Vibrio phage ICP1_2004_A
Expression system: Escherichia coli
UniProt:
  • Canonical: F1D5V7 (Residues: 1-248; Coverage: 100%)
Gene name: TUST1-10_00445
Sequence domains: CRISPR-associated protein (Cas_Csy2)
Csy3 Chains: C, D, E, F, G, H
Csy4 Chain: I
DNA (33-MER) Chain: Q
DNA (27-MER) Chain: R
RNA (59-MER) Chain: P

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.54Å
Relevant EMDB volumes: EMD-36831
Expression system: Escherichia coli